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A quantitative Streptococcus pyogenes-human protein-protein interaction map reveals localization of opsonizing antibodies.

Type Information
Nr 67 (Research article)
Authors Happonen, Lotta; Hauri, Simon; Svensson Birkedal, Gabriel; Karlsson, Christofer; de Neergaard, Therese; Khakzad, Hamed; Nordenfelt, Pontus; Wikström, Mats; Wisniewska, Magdalena; Björck, Lars; Malmström, Lars; Malmström, Johan
Title A quantitative Streptococcus pyogenes-human protein-protein interaction map reveals localization of opsonizing antibodies.
Journal Nat Commun (2019) 10 2727
DOI 10.1038/s41467-019-10583-5
Citations 9 citations (journal impact: 13.69)
Abstract A fundamental challenge in medical microbiology is to characterize the dynamic protein-protein interaction networks formed at the host-pathogen interface. Here we generate a quantitative interaction map between the significant human pathogen Streptococcus pyogenes and proteins from human saliva and plasma obtained via complementary affinity-purification and bacterial-surface centered enrichment strategies and quantitative mass spectrometry. Perturbation of the network using immunoglobulin protease cleavage mixtures of different concentrations of saliva and plasma and different S. pyogenes serotypes and their isogenic mutants reveals how changing microenvironments alter the interconnectivity of the interaction map. The importance of host immunoglobulins for the interaction with human complement proteins is demonstrated and potential protective epitopes of importance for phagocytosis of S. pyogenes cells are localized. The interaction map confirms several previously described protein-protein interactions however it also reveals a multitude of additional interactions with possible implications for host-pathogen interactions involving other bacterial species.